Fig. 4: Characteristics of AML mice treated with NAC(CR + NAC) or not(CR) and the normal mice treated with NAC (AD + NAC) or not (AD) and molecular signatures of CR EPCs.

a Representative H&E staining images (scale bar=10 μm) of BM sections in murine femurs, representative images of BM ECs in murine femurs stained with anti-EMCN antibody (red arrow) (scale bar = 10 μm) and CD117 antibody (blue arrow) (scale bar = 10 μm), and representative images of SPs stained with CD117 antibody (blue arrow) (scale bar = 10 μm). b Ratio of GFP-expressing AML-ETO primary mouse AML cells in BM nucleated cells. c Platelet (PLT) counts of all the mice in each group. d The frequencies of lineage^-cKIT⁺SCA1⁺ hematopoietic stem progenitor cells (HSPCs). e The frequency of myeloid cells within murine CD45⁺ BM cells was analyzed by flow cytometry. f The frequency of CD31⁺VE-cadherin⁺ ECs within the CD45^-Ter119^- BM cells from mice given the indicated treatments was analyzed by flow cytometry. g Quantification of intracellular ROS levels in BM ECs among all the mouse groups. b–g Six paired samples from each of the murine groups (N = 6 per group, n = 1 per sample) were collected and analyzed at indicated days. h Quantification of damaged BM ECs in murine femurs stained with an anti-EMCN antibody (Three paired samples from each of the murine groups (N = 3 per group, n = 1 per sample) were collected and analyzed at indicated days). i Heatmap and hierarchical clustering of RNA-seq data for the 12 libraries using the Euclidean distance. j Principal component analysis (PCA) score plot of 12 libraries of HD EPCs (N = 4), AML EPCs (N = 4) and AML CR EPCs (N = 4) at 7 days of culture. k Volcano plots of the upregulated genes (red) and downregulated genes (green) of HD EPCs and AML CR-EPCs. Gene set enrichment analysis (GSEA) comparing AML CR-EPCs and HD-EPCs for genes involved in l the blood vessel remodeling pathway, m the hematopoietic cell lineage pathway, n the reactive oxygen species pathway, o the P53 pathway and p the apoptosis pathway. i–p Four paired samples from each of the groups (N = 4 per group, n = 1 per sample) were collected on different days, and analyzed on the same day. N represents biological replicates; n represents technical replicates. Statistical analyses were performed using paired t tests. The data are presented as the means ± SEMs. The statistical tests were two-sided and no adjustments were made for multiple comparisons. Source data are provided as a Source Data file.