Fig. 4: Concentration-response chemoproteomics experiment.
From: Robust proteome profiling of cysteine-reactive fragments using label-free chemoproteomics
a The structures of four compounds that showed a range of promiscuity levels in the initial screen. These four compounds, along with the four compounds that showed specific interactions with a protein target (Fig. 3), were tested in a 10-point concentration-response experiment in HEK293T lysate (n = 4 for compound-treated samples; n = 25 DMSO control samples). The total number of liganding events detected for each of these eight compounds varied widely across the concentration range tested (b, c); p-values were calculated using Welch’s t-test (two-sided). d The concentration-response experiment was analysed by performing logistic regression to identify any concentration-dependent interactions between each compound and all detectable cysteine residues. e Heatmap showing the pTE50 values of all concentration-dependent interactions that were confidently identified in this experiment. The selective interactions that were identified in the initial screen are highlighted by black boxes. Source data are provided as a Source Data file.
