Fig. 1: Integration of published NSCLC and melanoma datasets. | Nature Communications

Fig. 1: Integration of published NSCLC and melanoma datasets.

From: A minimal gene set characterizes TIL specific for diverse tumor antigens across different cancer types

Fig. 1

A Study schema. B UMAP projection of the expression profiles of 253,161 CD8+ T cells from melanoma (n = 3 tumors) and NSCLC (n = 15 tumors, 12 normal lung). Immune cell subsets, defined by 14 unique clusters, are annotated and marked by color code. C Expression of selected genes including T cell marker genes (CD8A, CD4), tissue resident genes (ITGAE, ZNF683), stem related genes (IL7R, TCF7, CCR7 and SELL), tumor reactive genes (CXCL13 and ENTPD1), effector related genes (GZMK, GZMB, PRF1, GNLY and IFNG) and immune checkpoint genes (PDCD1, CTLA4, TIGIT, HAVCR2 and LAG3). D MANA-specific (red), MAA-specific (green), influenza-specific (blue) and EBV-specific (purple) clonotypes as identified in the cited publications were overlaid on the CD8+ UMAP. E Proportion of MANA-specific T cells in tumor tissues (tumor bed for melanoma) by cell type for six patients (3 NSCLC and 3 melanoma) with more than 30 functionally validated MANA-specific CD8+ TIL identified. Figure 1A was created in BioRender. Smith, K. (2024) https://BioRender.com/n17a163.

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