Fig. 5: Impact of the TME on pTRC functional programming.
A Boxplot of the average overall MANAscore (\(\sqrt{{{{\rm{MANAscore}}}\_{{\rm{i}}}}^{2}+{{{\rm{MANAscore}}}\_{{\rm{ni}}}}^{2}}\)) in paired adjacent normal lung and tumor (n = 11), comparison was performed using two-sided Wilcoxon signed-rank test. B Violin plot of the ratio of frequency of tumor: normal for paired pTRC (n = 156) and non-pTRC (n = 2748) clones. pTRC were identified in the adjacent normal lung by tracking TCR Vβ CDR3 clones corresponding to pTRC in the tumor. Only cells with a TCR Vβ CDR3 clones shared among tumor and adjacent normal lung were included in this analysis. C Average checkpoint, D cytotoxicity scores and (E) TRM scores of shared pTRC clones (n = 156) in normal and tumor tissues. F Mean expression of ENTPD1 by pTRC in adjacent normal lung and tumor tissue. Comparisons in B-F were performed with two-sided Wilcoxon rank-sum test. Box plots represent minima maxima as whiskers, the median as the center line within each box, and the interquartile range (IQR) between 25th and 75th percentiles as the bounds of each box.
