Fig. 6: A blind study to screen animal patients with an orthotopic liver tumor.
From: NIR-II-excited off-on-off fluorescent nanoprobes for sensitive molecular imaging in vivo
Schematic illustration of the fluorescence change of (a) NDPs and (b) 2S-NDPs in the presence of H2S and ROS. Created in BioRender. Jiajing, G. (2022) BioRender.com/m53h190. c The Flu.Final/Flu.Initial in liver at 10 h post i.v. injection of NDPs (10 mg Kg−1) in healthy mice (n = 4 mice). d Schematic illustration of the stratification of a patient group based on NIR-II fluorescence signal intensity in a blind study. Created in BioRender. Jiajing, G. (2022) BioRender.com/m53h190. e NIR-II fluorescence imaging of six mice at 10 h post i.v. injection of NDPs (10 mg Kg−1). (f) Stratification based on established diagnostic threshold and NIR-II fluorescence turn-on of six mice after i.v. injection of NDPs. The values of the first batch of mice have been marked with stars to distinguish them from the second batch (spheres). g Representative ex vivo NIR-II fluorescence images of main organs resected from the NDPs-treated ‘possible health mice’ group and ‘suspected orthotopic liver tumor mice’ group. h Representative ex vivo NIR-II fluorescence images of main organs resected from the 2S-NDPs-treated the ‘conformed orthotopic liver tumor mice’ group. i Representative macroscopic images of the liver tissues resected from (g, h), respectively. j The tumor-to-normal liver tissue signal ratio of NDPs-treated ‘suspected orthotopic hepatoma mice’ and 2S-NDPs-treated ‘conformed orthotopic liver tumor mice’, (n = 6 biologically independent samples for NDPs; mean ± SD, n = 3 biologically independent samples for 2S-NDPs; mean ± SD). Representative H&E staining of the liver tissue slice of the (k) ‘possible health mice’ group and the (l) ‘suspected orthotopic hepatoma mice’ group.
