Fig. 4: ZIP13 deficiency and overexpression affected the iron content of mitochondria and lysosome.

a Levels of iron in lysosome of WT and Zip13-KO MEFs. Iron stained by iron probe, and lysosome marked by lysoTracker green DND-26 (left). Green: lysosome; Magenta: Iron. Scale bar, 50 μm. The level of lysosome iron was quantified (right) (n = 10, biologically independent replicates). b Levels of iron in lysosome of WT and hZIP13 overexpression MEFs. Green: lysosome; Magenta: Iron. Scale bar, 50 μm. The level of lysosome iron was quantified (right) (n = 7, biologically independent replicates). c Mitochondrial iron in WT and Zip13-KO MEFs indicated by Rhodamine B-[(1,10-phenanthroline-5-yl)-aminocarbonyl] benzyl ester (RPA). Ferrous iron quenches RPA fluorescence. The RPA fluorescence intensity is inversely proportional to the mitochondrial LIP. Scale bar, 50 μm. The fluorescence intensity was quantified (right) (n = 10, biologically independent replicates). d, e Mitochondrial iron in MEFs indicated by RPA. Iron is indicated by fluorescence difference before and after PIH treatment (n = 3, biologically independent replicates). f Mitochondrial iron were detected in WT and Zip13-KO MEFs after treated with or without 50 μM brefeldin A (BFA) for 6 h (n = 3, biologically independent replicates). g Levels of total iron in WT and Zip13-KO MEFs were indicated by ICP-MS (n = 3, biologically independent replicates). h Levels of total iron in WT and hZIP13 overexpression MEFs (n = 4, biologically independent replicates). Data are mean ± SD. Statistical analysis was performed using two-tailed student’s t-test (a–h). Source data are provided as a Source Data file.