Fig. 4: Identification and validation of LAMC2 and TGM3 as biomarkers.

A Strategy used to select, rank, and validate candidates. The distribution of candidate ranks is shown with the top-scoring candidates labeled. B Example IHC images from HNSCC tissue microarray (TMA) cohort showing scores for LAMC2 and TGM3 along with normal tissue staining pattern and pan cytokeratin (panCK) stain used to identify epithelial cells. The scale bar is 200 µm. C–F Kaplan-Meier survival curves of DSS between patients in the TMA cohort based on (C) median-dichotomized LAMC2 intensity and (D) median-dichotomized LAMC2 intensity and N category risk groups (different letters indicate FDR < 5% for pairwise comparisons, generalized linear hypothesis test (two-sided) on multivariate Cox model). E median-dichotomized TGM3 normal-like staining, and (F) median-dichotomized TGM3 normal-like staining and N category risk groups (different letters indicate FDR < 5% for pairwise comparisons, generalized linear hypothesis test (two-sided) on multivariate Cox model). Unadjusted p-values on Kaplan-Meier curves are from a two-sided log-rank test. Shaded boundaries represent 95% confidence interval estimates. TMA results and clinical data are provided in Supplementary Data Table 4. Source data are provided as a Source Data file.