Fig. 8: Metabolic studies on leukemic cell lines (KG-1 and MOLM-13) or CD34⁺ AML cells isolated from AML patients in coculture with vehicle (PBS) or EV^AML for 24 h.

a–d Seahorse XFp Cell Mito Stress profile and analyses of KG-1 (n = 7 vehicle vs. n = 11 with EV^AML) or MOLM-13 cells (n = 8 vehicle vs. n = 14 with EV^AML); a–c Oxygen consumption rate (OCR) for KG-1 (a) or MOLM-13 (c). Bioenergetic parameters extracted from the OCR plot: basal respiration (basal), maximal respiration (Max Resp), spare respiratory capacity (SRC), and ATP-linked OCR (ATP prod) for KG-1 (b; p = 0.03) and MOLM-13 cells (d; p = 0.04). e, f Seahorse XFp Cell Mito Stress profile of AML CD34⁺ cells and relative bioenergetic parameters extracted from the oxygen consumption rate (OCR) plot (n = 4 independent biological experiments; p = 0,001 and p = 0.02). g, h Metabolic profile using SCENITH in MNCs cocultured with vehicle (PBS) or EV^AML and then stained for CD34⁺. Stacked graph with percentages of CD34⁺ cells using glucose dependence (gluco dep) or FAAO capacity on the left and glycolytic capacity or mitochondrial dependence (percentages) on the right (n = 4 vehicle vs. n = 6 with EV^AML; p = 0.02) from 4 biological experiments. Statistical significance was reported by two-way ANOVA with Sidak’s multiple comparisons test or using the Mann–Whitney test for unpaired samples. Data are presented as mean values ± SEM. Source data are provided as a Source Data file.