Fig. 4: SLPs mask TLR2 ligands of L. crispatus and prevent TLR2-dependent pro-inflammatory pathways activation. | Nature Communications

Fig. 4: SLPs mask TLR2 ligands of L. crispatus and prevent TLR2-dependent pro-inflammatory pathways activation.

From: Lactobacillus crispatus S-layer proteins modulate innate immune response and inflammation in the lower female reproductive tract

Fig. 4

a SDS-PAGE gel of crude SLPs extracted with LiCl 5 M from L. crispatus and L. iners isolates. 10μg total proteins were loaded per lane. Representative of three independent experiments. b HEK-TLR2 cells were stimulated for 16 h with bacteria (MOI = 10) and NF-κB activation was determined by measuring alkaline phosphatase activity and reading O.D. at 630 nm. c VK2 vaginal epithelial cells were stimulated for 16 h and IL-8 release in the supernatant was quantified by ELISA. d HEK TLR2 cells were stimulated with lithium chloride-treated bacteria (MOI = 10) for 16 h and IL-8 release in the supernatant was quantified by ELISA. TLR1, TLR2 and TLR6 dependence was investigated by pre-incubating cells for 30 min at 37 °C with 1 μg/ml of anti-TLR1, anti-TLR2, anti TLR6 or mIgG1 and mIgG2b isotype control antibodies. b–d A representative figure of three independent experiments is shown (mean +/− SD). Two-way ANOVA, Tuckey multiple comparison test. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0,001. Source data are provided as a Source Data file.

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