Fig. 5: SLPs isolated from L. crispatus vaginal strains are ligands of DC-SIGN. | Nature Communications

Fig. 5: SLPs isolated from L. crispatus vaginal strains are ligands of DC-SIGN.

From: Lactobacillus crispatus S-layer proteins modulate innate immune response and inflammation in the lower female reproductive tract

Fig. 5

a Western blotting results for DC-SIGN-Fc binding to purified SLPs isolated from vaginal L. crispatus strains (one representative of three replicates), b Purified SLPs isolated from vaginal L. crispatus strains (1 μg per well) were coated in 96-well plates and tested for their capacity to bind DC-SIGN-Fc. DC-SIGN-Fc (1 μg/ml) was pre-incubated or not with 20 mM EDTA or 40 mM glucose, mannose and galactose and allowed to react with the SLPs for 2 h at RT. Bound proteins were detected using a biotin-conjugated anti-IgG Fc specific antibody and avidin-HRP and reading O.D. at 450 nm. Data are mean +/−SD (n = 3 biological replicates). Source data are provided as a Source Data file.

Back to article page