Fig. 6: SLPs are detected in cervico-vaginal fluids. | Nature Communications

Fig. 6: SLPs are detected in cervico-vaginal fluids.

From: Lactobacillus crispatus S-layer proteins modulate innate immune response and inflammation in the lower female reproductive tract

Fig. 6

a Bacterial composition of the cervico-vaginal fluid samples determined by 16S rRNA gene sequencing, b Western blotting results for anti-Surface Layer Protein antibody binding to purified cervico-vaginal fluid samples. Representative of two technical replicates. Cervicovaginal concentrations of IL-1β (c), IL-8 (d), IL-6 (e) are shown from CST I samples (n = 26 women). Statistical analysis was performed using a one-sided Mann–Whitney test. Data are presented as median values and interquartile ranges (25th and 75th percentiles). Limit of detection is presented as dotted line. Cervicovaginal concentrations of IL-1β (f), IL-8 (g), IL-6 (h) are shown from CST III samples (n = 18 women). Statistical analysis was performed using a one-sided Mann–Whitney test. Data are presented as median values and interquartile ranges (25th and 75th percentiles). i Immunoprecipitation results for DC-SIGN-Fc binding to SLPs in CVF samples. Representative of three technical replicates. Source Data are provided as a Source Data File.

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