Fig. 7: INSIHGT reveals previously undescribed intercellular filaments traversing the Bowman space in mouse kidneys.

a Original image of multiplexed image of whole mouse glomerulus with full Bowman capsule. WGA Wheat germ agglutinin, PHA-E Phaseolus vulgaris hemagglutinin, GSL-I Griffonia simplicifolia lectin I. b Segmentation of microfilaments with en bloc preservation of native morphologies and spatial relationships in 3D Euclidean space. c Global representation of the 3D spatial distribution of microfilaments across the entire Bowman space. d Distinct and protean morphologies of the podocyte-to-parietal epithelial cell (PEC) microfilaments. e Schematic representation of reachers (contacting PEC surface) and stayers (remaining in the Bowman space) originating from podocyte surfaces with the related physical parameters. f Descriptive statistics of microfilament subtypes per analyzed glomeruli. N = 14 glomeruli analyzed across four mice (where three mice has four glomeruli imaged, one mouse has two glomeruli imaged, and the results were plotted together). g Correlation between the path length of each microfilament and the geodesic distance between its podocytic attachment point and its nearest neighbor. The data points were color-coded based on their glomerulus of origin in the dataset. Source data are provided as a Source Data file.