Fig. 8: INSIHGT enables non-destructive characterization and analysis of human clinical samples.

a A 3.5mm-thick human cerebellum triplex-stained for glial filaments (GFAP), neurofilament (NF-H) and blood vessels (G. simplicifolia lectin I). Orientation and coherence (or fractional anisotropy) visualization of neurofilament (b, NF-H) and glial filament (c, GFAP) via structure tensor analysis. d Enlarged view of the boxed area in a via post-hoc confocal microscopy. e Prototypical morphology of human cerebellar neurofilament inclusions, where their extensions may loop back to the Purkinje layer and occasionally to another inclusion body (lowest image). f Overview of the 1078 manually traced neurofilament inclusions across the cerebellar sample, color-coded by z-depth. Traditional 2D histology with special stains on pre-INSIHGT and post-INSIHGT processed samples. g H&E staining of human brain, h Left to right: Periodic acid-Schiff (PAS), Alcian blue, and Masson’s trichrome staining of human kidney, mouse colon, and mouse kidney sections respectively. i The Next-Generation Histopathology Pathway. INSIHGT is compatible with traditional histological pipelines, empowering a multi-pronged approach to maximizing the information extracted from clinical samples.