Table 1 Comparing NaP-TRAP to existing translation-based MPRA methods

From: NaP-TRAP reveals the regulatory grammar in 5’UTR-mediated translation regulation during zebrafish development

Method

System

Input

Specialized Equipment

Multi-Frame

Frame-specific

Translation Read-out

Growth-selection

Cell lines

DNA

None

No

Yes

Steady-State

FACS

Cell lines

DNA / RNA

Flow-cytometer

No

Yes

Steady-State

DART

Cell lysate

RNA

Ultracentrifuge

No

No

Equilibrium

Polysome profiling

Model organisms

DNA / RNA

Ultracentrifuge

No

No

Instantaneous

TRAP-seq

Model organisms

DNA / RNA

None

No

No

Instantaneous

NaP-TRAP

Model organisms

DNA / RNA

None

Yes

Yes

Instantaneous

  1. NaP-TRAP was developed to quantify the translation of thousands of reporters simultaneously in a frame-specific manner. In contrast to existing methods, NaP-TRAP can be adapted to dynamic model systems (e.g. the developing zebrafish embryo) and does not require specialized equipment or a large amount of source material to measure translation.
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