Fig. 7: T. brucei genome form widespread inter-chromosomal interactions with centromere clustering.
From: Inter-chromosomal transcription hubs shape the 3D genome architecture of African trypanosomes
a Top: Virtual 4C interaction profiles based on Micro-C data (10 kb resolution) showing the interaction frequencies of a subset of centromeres used as viewpoints (black arrowheads and green background) with the Tb427 genome version 12, haplotype B. Significant interactions as calculated using a two-sample KS-test were marked below each track (pink: p < 0.05; red: p < 0.01). Bottom: Each chromosome is displayed with the core region in white and subtelomeric regions in light gray. Centromeres are marked by red rectangles; rDNA loci are marked in green. b Fluorescence in situ hybridization (FISH)-based quantification of CIR147 repeats (located on chromosomes 4, 5 and 8) clusters (N = 1; analyzed nuclei: 171). Cells displaying no signal were excluded. c Model of T. brucei genome organization. T. brucei chromosomes make frequent centromere-centromere interactions, interactions between RNAPII TSSs, the active VSG gene and the splice leader locus, rRNA genes and some tRNA genes (not displayed).
