Fig. 3: Oscillations in cytosolic NADP and NAD redox state accompany the yeast metabolic cycle (YMC). | Nature Communications

Fig. 3: Oscillations in cytosolic NADP and NAD redox state accompany the yeast metabolic cycle (YMC).

From: A family of NADPH/NADP+ biosensors reveals in vivo dynamics of central redox metabolism across eukaryotes

Fig. 3

a Photograph of the coupled fermentor–fluorimeter setup used to monitor redox changes in YMC-synchronised cultures. b Diagram illustrating the coupled metabolic and cell division cycles observed during the YMC. CDC (cell division cycle), HOC (high oxygen consumption), and LOC (low oxygen consumption). c Representative traces showing the changes in dissolved oxygen, NAPstar4.3 (NADP redox state), Peredox (NAD redox state), and Hyper7 (H2O2) during two complete cycles of the YMC (n = 2, in which probe dynamics were measured for multiple YMC cycles in two independent YMC-synchronised cultures; Supplementary Fig. 7).

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