Fig. 7: Immunofluorescence staining analysis of the POF wounds in different groups. | Nature Communications

Fig. 7: Immunofluorescence staining analysis of the POF wounds in different groups.

From: Surface enzyme-polymerization endows Janus hydrogel tough adhesion and regenerative repair in penetrating orocutaneous fistulas

Fig. 7

a, b Representative immunofluorescence images for IL-6, α-SMA, CD31 and PCNA staining in different groups. Green, IL-6 or α-SMA or CD31; red, PCNA; blue, DAPI. Scale bar: 100 μm. c–e Quantitative analysis of IL-6, α-SMA, CD31 and PCNA fluorescence intensity in different groups. f Representative immunofluorescence images for 4-HNE and 8-OHdG staining in different groups. Red, 4-HNE or 8-OHdG; blue, DAPI. Scale bar: 100 μm. g Representative immunofluorescence images of iNOS and CD206 in different groups. Green, iNOS; red, CD206; blue, DAPI. Scale bar: 100 μm. h Quantitative analysis of 4-HNE and 8-OHdG positive regions in different groups. i Quantitative analysis of iNOS and CD206 positive regions in different groups. Data are shown as mean ± s.d. (n = 3 New Zealand rabbits in c–e, h, i). One-way ANOVA followed by Tukey’s multiple comparison test was used for statistical analysis of (c–e, h, i).

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