Fig. 2: The polar localization of OsGLS1 at the plasma membrane is required for its effect on root growth angle.
From: A root system architecture regulator modulates OsPIN2 polar localization in rice
Localization of various GLS1-GFP variants (green fluorescence) and FM4-64 staining (magenta fluorescence) in cells of the primary root cells from gls1-2 GLS1pro:GLS1-GFP (GLS1-GFP; a), gls1-2 GLS1pro:GLS1S30A-GFP (GLS1S30A-GFP; c) and gls1-2 GLS1pro:GLS1S30D-GFP (GLS1S30D-GFP; e) transgenic lines. Quantitative analysis of the fluorescence intensity of GFP and FM4-64 along the white dashed arrow in a (b), c (d) and e (f). S indicates the starting point of fluorescence intensity measurement. White arrowheads mark the polarity of GLS1-GFP; black arrows indicate the direction of root tips in a, c and e; arb. units, arbitrary units. Root phenotypes (g) and root growth angle (θrga; h) of the wild-type HJ2, the gls1-2 mutant and GLS1S30A-GFP and GLS1S30D-GFP transgenic lines grown on germination paper for 7 days after germination. Scale bars, 20 μm in a, c, e and 3 cm in g. In h, data represent means ± SD (n = 12); different letters indicate significant differences (p < 0.01; one-way ANOVA with Tukey’s honestly significant difference test).
