Fig. 3: Mutation of OsGLS1 affects auxin distribution and the polar localization of OsPIN2.
From: A root system architecture regulator modulates OsPIN2 polar localization in rice
GUS staining in the root tips of 7-day-old DR5:GUS seedlings in the XS63 and gls1 backgrounds growing vertically (a) and 2 h after reorientation by 90°. The black arrow indicates the direction of gravity (g; b). Localization of PIN2-GFP (green fluorescence) and FM4-64 (magenta fluorescence) in the primary root tip from 7-day-old seedlings of PIN2pro:PIN2-GFP in XS63 (c) and gls1 PIN2pro:PIN2-GFP (e). The black arrows indicate the direction of root tips. Quantitative analysis of fluorescence intensity along the white dashed arrows in c (d) and in e (f). S refers the starting point of fluorescence intensity measurement. g Immunostaining of OsPIN2 in the root tips of 5-day-old XS63 (left) and gls1 (right) seedlings using an antibody against OsPIN2 (anti-PIN2; red fluorescence). ep, epidermis; ex, exodermis; scl, sclerenchyma. The white arrowheads indicate PIN2 polarity at the plasma membrane. h Percentage of cells with apical, non-polar or basal OsPIN2 localization in epidermis, exodermis and sclerenchyma cells of XS63 and gls1 as revealed by immunostaining (means ± SD; n = 4). Scale bars, 100 μm in a and b, 20 μm in c, e and g.
