Fig. 1: RmlT transfers rhamnose from TDP-rhamnose to naked WTAs. | Nature Communications

Fig. 1: RmlT transfers rhamnose from TDP-rhamnose to naked WTAs.

From: Molecular properties of the RmlT wall teichoic acid rhamnosyltransferase that modulates virulence in Listeria monocytogenes

Fig. 1

a Electrophoretic mobility shift assay of the reaction mixture containing naked WTAs and TDP-rhamnose, in the absence (−) and presence (+) of RmlT. The individual bands observed in each lane correspond to different WTAs chains. b Coupled functional assay quantifies the amount of phosphate released after hydrolysis of TDP molecules generated by RmlT. In both assays reactions ran for 60 min with 5 mM WTAs, 1 mM TDP-rhamnose and 10 µM RmlT. Mean ± SD (n = 3 refers to biological replicates) and individual measurements are shown; one-way ANOVA; ****p < 0.0001. Source data are provided as a Source Data file.

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