Fig. 2: Structure of L. monocytogenes RmlT.
a Cartoon representation of a subunit of RmlT (PDB code: 8BZ4; chain A) coloured from pink to purple, with subdomains and N and C termini indicated. Interaction between catalytic and oligomerization subdomains is indicated by dashed rectangle. b Superposition of RmlT (blue, PDB code: 8BZ4, chain A) and S. aureus TarS27 (pink, PDB code: 5TZ8) and TarP24 (yellow, PDB code: 6H1J) through catalytic domain (wheat ellipse) Cα atoms in helices h5, h6 and h11 (RmlT: residues 91–101, 118–130, 196−206; TarS: residues 72–82, 99–111,176–186; TarP: residues 73–83; 100–112; 179–189) with RMSD values of 1.185 Å (TarS) and 0.886 Å (TarP). c Two views of RmlT dimer showing subunits coloured blue and white and dimerization interface residues Y347, Y368 and E372 as spheres. d Quantification of fluorescence associated with gp17-GFP bound to rhamnose at the surface of the L. monocytogenes wild-type EGDe strain and mutant strains EGDe ΔrmlT::rmlT, EGDe ΔrmlT::rmlTY368A+E372A and EGDe ΔrmlT::rmlTY347A+Y368A+E372A. Mean ± SD (n = 3 refers to biological replicates) and individual measurements are shown; one-way ANOVA, **** = p < 0.0001. e Fluorescence microscopy images of L. monocytogenes cells stained with wheat germ agglutinin (WGA, red) and gp17-GFP (green) which binds to rhamnose at the bacterial surface. Source data are provided as a Source Data file.
