Fig. 3: Using TDF sensor to image circSCMH1 in cultured neurons.

A CCK8 assay was used to detect the cell viability of HT-22 cells under TDF sensor incubation for 0, 3, 6, and 12 h. Data are presented as mean ± SEM, n = 6 biological replicates/group. B Schematic diagram of the approach for monitoring circSCMH1 in HT-22 cells using a live cell station. Created in BioRender. Yao, H. (2024) BioRender.com/v74m668. C Images of circSCMH1 in HT-22 cells after incubation with TDF sensor for 3, 6, and 12 h. Red, circSCMH1. Scale bar, 20 μm. D Quantification of circSCMH1 intensity in HT-22 cells. Data are presented as mean ± SEM, n = 6 images (selected 5 representative cells in each image for quantification)/group. ^**P = 0.0016 versus 3 h using one-way Friedman test followed by the Dunn’s multiple comparisons test. E Schematic diagram of the approach for monitoring circSCMH1 in HT-22 cells after 2-DG treatment using a live cell system. Created in BioRender. Yao, H. (2024) BioRender.com/v74m668. F Images of circSCMH1 in HT-22 cells after treatment with 2-DG and incubation with TDF sensor for 12 h. Red, circSCMH1. This experiment was repeated at least three independent experiments. Scale bar, 20 μm. G Quantification of circSCMH1 intensity in HT-22 cells under control and 2-DG treatment condition. Con, control; 2-DG, 2-Deoxy-D-glucose. Source data are provided as a Source Data file.