Fig. 4: Monitoring circSCMH1 in cerebral cortex of freely moving stroked mice.

A Schematic diagram of the experimental design for monitoring circSCMH1 in cerebral cortex using miniaturized two-photon microscope. Mice were injected via tail vein with the TDF sensor at a concentration of 1 μM, with a volume of 100 μL per subject. Created in BioRender. Yao, H. (2024) BioRender.com/p66e150. B Representative images and quantification of circSCMH1 expression in cerebral cortex using miniaturized two-photon microscope from Pre-stroke and Post-stroke mice. Red, circSCMH1. Scale bar, 20  μm. C Quantitation of fluorescence intensities at different time point before and after stroke. n = 5 mice/group. Data are presented as mean ± SEM, ^***P = 0.0002 (1 day), ^***P < 0.001 (3 days), ^***P = 0.0003 (5 days), and ^**P = 0.0023 (7 days) versus the Pre group using one-way repeated measures ANOVA followed by Holm–Sidak post hoc multiple comparison test. WT wild type, Pre pre-stroke, TPM two-photon microscopy. Source data are provided as a Source Data file.