Fig. 5: In situ imaging of circSCMH1 in mouse brain sections.

A Schematic diagram of the experimental design for in situ imaging circSCMH1 in mouse brain sections. Created in BioRender. Yao, H. (2024) BioRender.com/w93y687. B Colocalization of NeuN and circSCMH1 in mouse brain sections after incubation with TDF sensor for 3, 6, and 12 h. Green, NeuN; Red, circSCMH1; Blue, DAPI. Scale bar, 20 μm. C Quantification of circSCMH1 intensity in neurons. Data are presented as mean ± SEM, n = 4 slices (selected 10 representative cells in each slice for quantification) from 4 mice/group. ^***P < 0.001 versus 3 h, ^###P < 0.001 versus 6 h using one-way ANOVA followed by Holm–Sidak post hoc multiple comparison test. D Schematic diagram of the experimental design for in situ imaging circSCMH1 in PT mouse brain sections. Created in BioRender. Yao, H. (2024) BioRender.com/w93y687. E Colocalization of NeuN and circSCMH1 in brain sections of Sham and PT mice after incubation with TDF sensor for 12 h. Green, NeuN; Red, circSCMH1; Blue, DAPI. Scale bar, 20 μm. F Quantification of circSCMH1 intensity in neurons. Data are presented as mean ± SEM, n = 4 slices (selected 10 representative cells in each slice for quantification) from 4 mice/group. ^***P < 0.001 versus Sham group using unpaired two-tailed Student’s t test. G The procedure of in situ imaging using biotin-labeled probes. H The procedure of in situ imaging using TDF sensors. I Representative images of the colocalization of NeuN and circSCMH1 in mouse brain sections using biotin-labeled probes and TDF sensors respectively. Green, circSCMH1; Red, NeuN; Blue, DAPI. Scale bar, 20 μm. J The plots were the analysis of circSCMH1 intensity, circSCMH1⁺ cells/mm², and signal-to-noise ratio in neurons. Data are presented as mean ± SEM, n = 4 slices (selected 10 representative cells in each slice for quantification) from 4 mice/group for the analysis circSCMH1 intensity, circSCMH1⁺ cells/mm², and signal-to-noise ratio. ^***P < 0.001 versus biotin-labeled probes using unpaired two-tailed Student’s t test. WT wild type, PT photothrombotic, LSCM laser scanning confocal microscope. Source data are provided as a Source Data file.