Fig. 7: Cryo-EM structure of PolD in complex with Rpa2WH.
From: Communication between DNA polymerases and Replication Protein A within the archaeal replisome
a Final composite map of PolD-Rpa2WH at 2.9 Å global average resolution. b PolD-Rpa2WH interface, with Rpa2WH residues colored according to the intensity ratio Icplx/Ifree from blue (weak attenuation) to red (large attenuation) as in Fig. 3l. c Superposition of the two different classes that resulted from a focused 3D classification with a soft mask around Rpa2WH, and d, e a detailed view of the interface between PolD and Rpa2WH for each class. f Schematic drawing of Rpa2WH with the secondary structure elements that comprise the PolD interface colored in blue. g, h Comparison of the rotational displacement difference between 3D classes 1 and 2, and alignments of the Primase-Rpa2WH structure to Rpa2WH in the PolD-Rpa2WH structure, showing the respective volumes of the resulting clashes in red.
