Fig. 8: Proposed model for the interaction of Primase and RPA during primer elongation.
From: Communication between DNA polymerases and Replication Protein A within the archaeal replisome
a Superposition of the PriSLΔCTD-Rpa2WH crystal structure with DNA-bound PrimPol (PDB ID 5L2X58), aligned to our previously reported structure of ssDNA-bound RPA trimeric core (PDB ID 8AAS22). The PrimPol-DNA ternary structure was used to model the DNA substrate in the PriSL active site. The RPA-ssDNA structure was oriented so that the C-terminal of the Rpa2 core would face the N-terminal of the Rpa2WH, while matching the 5’ and 3’ ends of the DNA template. b Schematic diagram of our proposed ‘WH-bait’ mechanism for WH domain-dependent PriSL stimulation by RPA. A potential polymerase switch event mediated by the PolD binding site in Rpa2WH is shown in step 4.
