Fig. 3: RNA cap coordination in T. brucei CBP20.
From: Structural basis of Spliced Leader RNA recognition by the Trypanosoma brucei cap-binding complex
A Cartoon representation of TbCBP20 of the TbCBC-trimer-cap4 sample in shades of yellow indicating the three domains of TbCBP20. The TbCBP30 peptide bound to the CTD of TbCBP20 is also shown, with contact residues in stick representation. The cap-ligand is shown as a stick model, and its flexibility is indicated. B Residues in the m7GTP binding pocket of T. brucei and (C) human CBP20, based on PDB:1H2T35. D Deep-EMhancer post-processed trimer cryo-EM map transparent with cartoon model and cap4-hexanucleotide in stick representation and at level 0.3 and (E) at a very reduced level of 0.03. F Electrophoresis mobility shift assay (EMSA) of TbCBC-dimer binding to the SL RNA exon with different 5’ chemistry. G EMSA of TbCBC-trimer binding to the SL RNA exon with different chemistry. EMSAs in (F) and (G) were run in triplicate reactions; the intensity of PAGE gel bands was normalized in relation to the protein-free RNA control band. A Hill model was used to fit the binding curve. Source data are provided as a Source Data file.
