Table 2 Summary of pharmacological parameters measured for apelin receptor variants

Variant	Expression		Fl. ligand binding		Radioligand binding			Function
Variant	Mem. eGFP (% WT)	Object count/ region	Mem. apelin647 (% WT)	Mem. ELA647 (% WT)	K_D (nM)	B_max (fmol/ mg)	Hill slope (nH)	pD₂	E_max (ΔPWV)
WT	100.00 ± 23.52	17.00 ± 6.88	100.0 ± 27.09	100.00 ± 19.10	0.21 ± 0.05	236.80 ± 27.07	1.26 ± 0.22	8.32 ± 0.24	39.48 ± 3.93
V/L38^1.42	121.40 ± 31.87	16.58 ± 7.99	94.74 ± 17.52	92.53 ± 28.43	NBD	NBD	NBD	7.67 ± 0.48	42.55 ± 15.16
G/X45^1.49	32.18 ± 16.67	7.67 ± 3.68	12.18 ± 6.48	20.73 ± 12.55	NBD	NBD	NBD	–	–
T/M89^2.64	104.94 ± 34.40	19.83 ± 8.67	82.95 ± 24.46	31.75 ± 13.20	0.25 ± 0.04	440.47 ± 38.36	1.55 ± 0.22	7.72 ± 0.21	50.06 ± 8.32
R/H168^4.64	119.93 ± 40.29	23.00 ± 10.04	2.53 ± 2.10	17.29 ± 9.42	NBD	NBD	NBD	n/a	5.49 ± 6.42
T/X227^5.64	12.38 ± 3.90	5.08 ± 1.51	2.94 ± 2.78	14.83 ± 7.02	NBD	NBD	NBD	–	–
Untrans.	0.00	0.00	0.00	0.00	NBD	NBD	NBD	n/a	0.21 ± 7.07

Colours indicate the missense (V/L38^1.42, blue; T/M89^2.64, red; R/H168^4.64, magenta) or frameshift (G/X45^1.49, T/X227^5.64, both yellow) variants.
Mem. membrane, Fl. ligand fluorescent ligand, ΔPWV change in peak wavelength value, WT cells transfected with wild-type apelin receptor, Untrans. untransfected cells.

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