Fig. 2: Cryo-EM structure of the VANGL1-PK1 complex.

a Cryo-EM map of the VANGL1-PK1 complex. The sharpened map is reconstructed with D3 symmetry shown at a contour level of 0.105. The VANGL1 densities are colored in blue and the extra densities are colored in grey. b Superposition of individual VANGL1 and the VANGL1-PK1 complex structures based on the top VANGL1 trimer. c Unsharpened cryo-EM maps of VANGL1 and the VANGL1-PK1 complex refined with C3 symmetry. Maps are colored by chains and the potential PK1 densities are colored in green. The contour levels of these maps are 0.350 (VANGL1) and 0.075 (VANGL1-PK1), respectively. d Surface electrostatic potential (in units of k_BT/e, where k_B is the Boltzmann constant, T is the absolute temperature and e is the elementary charge) of the VANGL1 trimer-trimer interface that interacts with PK1. e The ten positively charged residues on the potential PK1-binding interface of VANGL1 are shown with side chains.