Fig. 5: Sliding tether assay.
From: Intracellular pressure controls the propagation of tension in crumpled cell membranes
HEK293T cells were dyed with DiI-C12 and incubated in HEPES buffer with different NaCl concentrations, as indicated. Next, a membrane tether was pulled out of the cell and then the bead was moved sidewise. Data was acquired from 3 independent experiments. At 50 and 150 mM NaCl, the tether origin did not slide along the cell plane in spite of the bead movement (20 and 17 cells for 50 mM and 150 mM, respectively). At 0 mM NaCl in 4 out of 10 cells, the tether origin did not slide significantly, while in the remaining cells or in the experiments conducted on 3 GPMVs, the tether origin freely slid.
