Fig. 3: Measurement of NA duplex stability by thermal melting. | Nature Communications

Fig. 3: Measurement of NA duplex stability by thermal melting.

From: Counterintuitive DNA destabilization by monovalent salt at high concentrations due to overcharging

Fig. 3

Source data are provided as a Source Data file. a Representative melting curves obtained from a fluorescence quenching test. The top panels are the fluorescence intensity as a function of temperature, and the bottom panels are the derivative of fluorescent intensity versus temperature. b Melting temperature Tm as functions of \({c}_{{{{\rm{salt}}}}}\). The averages and standard errors from several independent experiments (six for NaCl and three for other ions) are plotted as data points and error bars. c Two different transitions in MT and thermal melting experiments.

Back to article page