Fig. 3: Increased PDAC lymphocyte infiltration is associated with increased activated Treg clonality.

a The clonality of intra-tumoural T cells within total CD4 and CD8 T cell populations, measured by percentage of clones consisting of >2 cells. Orange represents ME patients and grey represents AE patients. b Immune cell subset proportions between ME and AE patient groups within tumour T cell subsets as a proportion of total CD4 and CD8 T cells, respectively. c Clonality of the tumour T cell subpopulations between the ME and AE patient groups via two measures: (top) intra-subset clonality (the percentage of cells in clones >2 cells per subset, measuring the clonality within the subset thus reflecting specific cell populations which are actively expanding), and (bottom) inter-subset clonality (the percentage of cells of each cell type as members of clones >3 cells across all populations, demonstrating, this indicates cells within each T cell subset that may be members of larger clones than span multiple phenotypes, reflecting T cell plasticity of expanding clones). d Level of tumour TCR clonal sharing between (left) CD4 T cell and (right) CD8 T cell populations. Each line represents a sharing of TCR clones between cell types, and the line thickness denotes the mean relative level of sharing. A red line denotes that the clonal sharing between the corresponding cell types is significantly higher in the ME patients than AE, and a blue line denotes that the clonal sharing between the corresponding cell types is significantly lower in the ME patients than AE. The size of the dot represents the mean relative frequency of the corresponding cell type. All analyses in this figure were performed on the intra-tumoural B cells from PancrImmune dataset. * denotes p values < 0.05, and tests were performed by two-sided MANOVA. ME patients have an n = 5 and AE patients have an n = 7. Boxplots define the 10th, 25th, 50th, 75th and 90th percentiles.