Fig. 1: Cluster annotation and differences for cell types in single-cell RNA-Seq of maize primary root tips under HS.
From: Single-cell transcriptomes reveal spatiotemporal heat stress response in maize roots
A Overview of the maize scRNA-seq and cluster annotation workflow: Protoplasts were isolated from 4 mm root tips of maize under control and heat shock (42 °C for 2 h), respectively. ScRNA-seq libraries were generated using the 10x Genomics platform, followed by high-throughput sequencing. B Visualization of nine broad populations and their spatial distribution in maize root tips using UMAP. C Expression patterns of specific marker genes for nine cell types. Dot diameter represents the proportion of cluster cells expressing a given gene, while color indicates the mean expression across cells in these cell clusters. D RNA in situ hybridization of cell-type marker genes for the nine putative clusters and response to HS. Scale bars, 100 µm. The right panel of each gene indicates UMAP visualization of the expression of cell-type marker genes in the maize root tip. At least three repeats, each consisting of individual plants were performed for each gene.
