Fig. 3: Reclustering and the pseudotime trajectory of columella upon HS.
From: Single-cell transcriptomes reveal spatiotemporal heat stress response in maize roots
A Columella cells were reclustered to two putative subclusters. B Pseudotime analysis of two subtypes of columella cells, cells from the two subtypes were unequally distributed across the reconstructed trajectory. C Heatmap showing the differentially expressed genes in two branches during the differentiation trajectory between two subclusters of columella cells. Gene functions revealed by GO enrichment analysis for genes in the three clusters of expression patterns are shown at right. D Illustrative instances include an early (pre-branch), an earlier (columella1), and a late (columella2) expressed gene specific to columella cells. The gene expression in each cell is overlaid onto the UMAP cluster and trajectory, with deeper red colors signifying higher gene expression. E Distribution difference of cells along the pseudotime trajectory of columella cells under HS.
