Fig. 1: Vascular-secreted Endocan promotes proliferation and migration of GBM cells. | Nature Communications

Fig. 1: Vascular-secreted Endocan promotes proliferation and migration of GBM cells.

From: Endothelial-secreted Endocan activates PDGFRA and regulates vascularity and spatial phenotype in glioblastoma

Fig. 1

a Proliferation of GBM cells (711, 1079, 157, 1051) treated with control media or conditioned media collected from Tumor Endothelial Cells (TEC CM); n = 3 biological replicates, ***P < 0.0001 for 711 cells, *P = 0.015 for 1079 cells, ***P = 0.0001 for 157 cells, **P = 0.0007 for 1051 cells, unpaired two-tailed Student’s t- est. b Kaplan–Meier survival analysis of SCID mice intracranially injected with gliomaspheres alone (1051) or gliomaspheres with VE cells (1051 + TEC) at a 10:1 ratio. (n = 5 mice per group). **P = 0.0064, Log-rank (Mantel-Cox) test. c Volcano plot of RNA-sequencing (RNA-seq) data comparing gene expressions of normal and tumor endothelial cells, with ESM1 (red font). FC = 9.76, FDR corrected p = 0.0031.Original data was used from previously published study7. d ELISA assay comparing levels of secreted Endocan in CM from Normal Human Astrocytes (NHA), Human Brain Endothelial Cells (HBEC-5i), Tumor-associated Endothelial cells (TEC15, TEC14), and GBM cells (1079, 157, 711, and 1051), n = 2 biological replicates. e Representative immunofluorescence (IF) staining for Endocan (red) and CD31 (green) in formalin-fixed paraffin-embedded tissue sections from glioblastoma patient 1051. Nuclei stained with DAPI (blue). Scale bar, 50 μm. n = 3 different patients. f Proliferation of GBM cells (711, 1079, 157, 1051, 413) with or without rEndocan (10 ng/ml). Proliferation was measured 5 days after treatment; n = 4 biological replicates; ****P < 0.0001 for 711 cells, **P = 0.0043 for 1079 cells, ****P < 0.0001 for 157 cells, ****P < 0.0001 for 1051 cells, unpaired two-tailed Student’s t test. g Representative microscopic images of control and rEndocan treated (10 ng/ml) GBM spheroids encapsulated in the hydrogel. Images were taken 3 days post-encapsulation. Scale bar, 500 µm. h Quantification of migration distance in control and rEndocan (10 ng/ml) spheroids formed by 408, 413, or 1079 cells. n = 9 spheroids for 408 cells, n = 8 spheroids for 413 cells, and 4 spheroids for 1079 cells were used for analysis ****P < 0.0001 for 408 cells, **P = 0.0053 for 413 cells and ****P < 0.0001 for 1079 cells, unpaired two-tailed Student’s t test. Source data are available in the Source Data file.

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