Fig. 4: Endocan binds to and activates PDGFRA in human glioblastoma cells. | Nature Communications

Fig. 4: Endocan binds to and activates PDGFRA in human glioblastoma cells.

From: Endothelial-secreted Endocan activates PDGFRA and regulates vascularity and spatial phenotype in glioblastoma

Fig. 4

a Western blot (WB) analysis of samples obtained during pull down assay of His-tagged rEndocan and Fc-tagged rPDGFRΑ. Representative result of n = 3 biological replicates. b Competition binding assay of Endocan-Alexaflour488 and PDGF-BB-Alexaflour647. 1079 cells were incubated with indicated concentrations of both proteins and subsequently analyzed by flow cytometry, n = 3 biological replicates with similar results. c Western blot analysis of 1079 cells incubated with different concentrations of rEndocan or rPDGF-BB for 30 min. Representative result of n = 3 biological replicates. d Western blot analysis of 1079 cells incubated with 10 ng/ml rEndocan or rPDGF-BB (10 ng/ml) for the indicated period of time. Representative result of n = 3 biological replicates. e Heatmap showing significant (p < 0.05) normalized enrichment scores (NES) calculated from RNAseq data of 1079 cells treated with 10 ng/ml rEndocan or rPDGF-BB for 48 h, n = 4 biological replicates. Source data are available in the Source Data file.

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