Fig. 2: VLDLR is expressed in choroid plexus epithelial cells.

Staining for VLDLR and basement membrane (Type IV collagen, COL4) in the choroid plexus in C57BL/6 J mice (a, b) show clear expression of VLDLR in epithelial cells. b Magnified insert from (a). Epithelial cell nuclei indicated with white asterisks. c staining for choroid plexus marker aquaporin-1 (AQP1). d single cell RNAseq data (reproduced from¹⁴) showing expression of Vldlr and Lrp8 (gene encoding ApoER2) in different cell types in 2–3 month old C57BL/6 mouse brain. Choroid plexus epithelial cells (red) show Vldlr^high/Lrp8^low expression profile. e immunostaining in choroid plexus shows apical ApoER2 polarization in epithelial cells (indicated with arrows). f Immunostaining for VLDLR and ApoER2 in ventricle wall. CSF: cerebrospinal fluid. g single cell RNAseq violin plot (reproduced from ref. ³⁴) of Vldlr and Lrp8 in choroid plexus endothelial cells (EC) and epithelial cells (EpiC) in adult mice. Maximum (max), median (med) and third quartile (Q3) values are provided below the graphs.