Fig. 1: GmERF13s are induced by salt stress and highly expressed in nodules.
From: GmERF13 mediates salt inhibition of nodulation through interacting with GmLBD16a in soybean
a Heatmap of partial genes induced by 150 mM NaCl treatment with log2FC (fold change) >5. The color key represents gene expression (Transcripts per kilobase million, TPM). b Phylogenetic analysis of ERF13 genes in Glycine max, Arabidopsis thaliana, Lotus japonicus, and Medicago truncatula. The phylogenetic tree was constructed based on the coding sequences of ERF13 genes using MEGAX phylogenetic analysis software. Scale bar, 0.10. c Histochemical assays were conducted on transgenic seedling roots and nodules expressing ProGmERF13a:GUS, ProGmERF13b:GUS, ProGmERF13c:GUS, and ProGmERF13d:GUS following exposure to 150 mM NaCl for 0 h, 2 h, and 12 h. Three independent experiments were repeated with similar results. GUS activity was observed in both uninoculated roots and nodules after 21 dpi, which had been subjected to 150 mM NaCl treatment for 0 h, 2 h, and 12 h, respectively. Scale bar, 100 µm. d Heatmap of GmERF13s expression in different organs including Nodule, 48 hpi (hours post inoculation) Scrip Root, Root, Root Tip, 48 hpi_IN_RH (hours post inoculation in root hair), 48 hpi_UN_RH (hours post uninoculation in root hair), 12 hpi_IN_RH, 12 hpi_UN_RH, 24 hpi_IN_RH, 24 hpi_UN_RH, Flower, Green Pods, Apical Meristem, and Leaves. The data were downloaded from SoyBase (https://www.soybase.org/). Source data are provided as a Source Data file.
