Fig. 2: GmERF13s function as negative regulators in nodulation and nodule function. | Nature Communications

Fig. 2: GmERF13s function as negative regulators in nodulation and nodule function.

From: GmERF13 mediates salt inhibition of nodulation through interacting with GmLBD16a in soybean

Fig. 2

a Nodulation phenotypes of W82 and GmERF13 CRISPR/Cas9 mutants after 28 dpi (days post inoculation). Scale bar, 1 cm. b Nodule number/dry weight per plant of W82 and GmERF13 CRISPR/Cas9 mutants after 28 dpi. Data are presented as means ± SD from three biological repeats and 16 plant roots were collected for analysis in each repeat. Different letters indicate significant differences at P < 0.05 (One-way ANOVA) in multiple comparisons tests. c, d Total dry weight of nodules/nodule number c and nitrogenase activity of nodules d in W82 and GmERF13 CRISPR/Cas9 mutants after 28 dpi. Data are presented as means ± SD from three biological repeats. 18 plants were collected per repeat for average dry weight analysis of root nodules. For nitrogenase activity assessment, 0.2 g of nodules were collected from each sample. Each repeat included 10 parallel samples for each material. Different letters indicate significant differences at P < 0.05 (one-way ANOVA) in multiple comparisons tests. eh Relative expression levels of GmENOD40, e, f and GmENOD11 g, h by RT-qPCR in GmERF13 CRISPR/Cas9 mutants after inoculation or uninoculation for 7 days and 14 days. Data are presented as means ± SD from three biological replicates. Asterisks represent statistically significant differences relative to W82. Two-sided Student’s t test: *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001. The boxes in (bd) indicate the first and third quartiles, and the whiskers indicate the minimum and maximum values. The lines within the boxes indicate the median values. The exact P values are noted in the Source data. Source data are provided as a Source Data file.

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