Fig. 3: GmERF13s interact with GmLBD16a.
From: GmERF13 mediates salt inhibition of nodulation through interacting with GmLBD16a in soybean
a Y2H assays showing the interactions of GmLBD16a and GmERF13s in yeast cells. GmERF13 proteins were used as baits and GmLBD16a protein as prey. Empty vectors AD and BD were co-transformed as a negative control. 30 mM 3-amino-1, 2, 4-triazole (3-AT) was used in SD/-Leu/-Trp/-His/-Ade culture medium to inhibit self-activation. Three independent experiments were repeated with similar results. b BiFC assays showing the interactions of GmLBD16a and GmERF13s in tobacco leaf cells. The vectors containing GmLBD16a and GmERF13 (GmERF13a, GmERF13c, or GmERF13d) were co-expressed in tobacco leaf cells. After 3 days culture, lower epidermis leaves were used for YFP signals observation. Three independent experiments were repeated with similar results. Scale bar, 20 µm. c Co-IP assays showing the interactions of GmLBD16a and GmERF13s in tobacco leaf cells. One vector containing GmLBD16a and another vector containing GFP or GmERF13 (GmERF13a, GmERF13b, GmERF13c, or GmERF13d) were constructed, and both were used for tobacco leaf transformation. The proteins were extracted from tobacco leaf cells and immunoprecipitated by GFP beads. The input and co-immunoprecipitated proteins were subjected to immunoblot analysis with anti-MYC (upper panel) or anti-GFP antibody (lower panel). Three independent experiments were repeated with similar results. Source data are provided as a Source Data file.
