Fig. 4: GmERF13s antagonize GmLBD16a in regulating root nodulation.
From: GmERF13 mediates salt inhibition of nodulation through interacting with GmLBD16a in soybean
a Nodulation phenotypes were assessed in W82, GmLBD16aOX, and GmERF13bcCR mutant background plants, which expressed an empty vector (pS1300), GmERF13sOX, or GmLBD16aOX in their hairy roots after 28 dpi. Scale bar, 1 cm. b Nodule number per hairy root of W82, GmLBD16aOX, and GmERF13bcCR mutant background plants expressing an empty vector (pS1300), GmERF13sOX, or GmLBD16aOX in their hairy roots was assessed after 28 dpi. Data are presented as means ± SD from three biological repeats and 15 hairy roots were collected for analysis in each repeat. Different letters indicate significant differences at P < 0.05 (one-way ANOVA) in multiple comparisons tests. The boxes indicate the first and third quartiles, and the whiskers indicate the minimum and maximum values. The lines within the boxes indicate the median values. c Relative expression levels of GmERF13s and GmLBD16a were quantified by RT-qPCR in hairy roots of W82, GmLBD16aOX, and GmERF13bcCR mutant background plants, which expressed an empty vector (pS1300), GmERF13sOX, or GmLBD16aOX in their hairy roots after 28 dpi. Hairy roots were divided into three mixed samples for detecting, respectively. Data are presented as means ± SD from three biological replicates. Asterisks represent statistically significant differences relative to empty vector (pS1300). Two-sided Student’s t test: *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001. The exact P values are noted in the Source data. Source data are provided as a Source Data file.
