Fig. 5: GmEXP17s are positive regulators of nodulation in hairy root.
From: GmERF13 mediates salt inhibition of nodulation through interacting with GmLBD16a in soybean
a Nodulation phenotypes of empty vector (pS1300), GmEXP17sOX, and artificial microRNA (amiR; amiR-GmEXP17) transgenic hairy roots after 28 dpi. Scale bar, 1 cm. b Nodule number per hairy root of empty vector (pS1300), GmEXP17sOX, and artificial microRNA (amiR; amiR-GmEXP17) transgenic hairy roots after 28 dpi. Data are presented as means ± SD from three biological repeats and 19 plant roots were collected for analysis in each repeat. Different letters indicate significant differences at P < 0.05 (one-way ANOVA) in multiple comparisons tests. The boxes indicate the first and third quartiles, and the whiskers indicate the minimum and maximum values. The lines within the boxes indicate the median values. c Relative expression levels of GmEXP17s by RT-qPCR in transgenic hairy roots expressing empty vector (pS1300) and GmEXP17sOX after 28 dpi. All hairy roots of empty vector (pS1300) and GmEXP17sOX were divided into three mixed samples for detecting, respectively. Data are presented as means ± SD from three biological replicates. Asterisks represent statistically significant differences relative to empty vector (pS1300). Two-sided Student’s t test: *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001. d Relative expression levels of GmEXP17s by RT-qPCR in transgenic hairy roots expressing empty vector (pS1300) and amiR-GmEXP17 after 28 dpi. All hairy roots of empty vector (pS1300) and amiR-GmEXP17 were divided into three mixed samples for detecting, respectively. Data are presented as means ± SD from three biological replicates. Asterisks represent statistically significant differences relative to empty vector (pS1300). Two-sided Student’s t test: *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001. The exact P values are noted in the Source data. Source data are provided as a Source Data file.
