Fig. 8: Salt stress-induced expression of GmERF13s is ABA-dependent.
From: GmERF13 mediates salt inhibition of nodulation through interacting with GmLBD16a in soybean
a–e Seven-day-old seedlings were treated with 150 mM NaCl a–d, 150 mM NaCl with 100 µM ABA inhibitor (Fluridone) a–d, and 10 µM ABA e respectively. Roots were collected at 0 h, 6 h, 12 h, and 24 h after treatment for expression analysis of GmERF13s by RT-qPCR. GmActin was used as an endogenous reference for gene expression analysis. Data are presented as means ± SD from three biological replicates. f Relative expression levels of GmERF13s determined by RT-qPCR in transgenic hairy roots expressing empty vector (pS1300) or GmABIsOX after 28 dpi. Data are presented as means ± SD from three biological replicates. Asterisks represent statistically significant differences from empty vector (pS1300). Two-sided Student’s t test: *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001. g Luciferase activity assays using soybean leaves protoplasts transferred co-expressing constructs empty vector (pBI221)/ProGmERF13s-LUC and 35S:GmABIs/ProGmERF13s-LUC. Relative LUC/REN ratios were determined by measuring LUC and REN activities of leaves protoplasts co-expressing constructs. Data are presented as means ± SD from three biological repeats. Asterisks represent statistically significant differences from the empty vector (pBI221)/ProGmERF13s-LUC. Two-sided Student’s t test: *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001. h–k ChIP-qPCR assays of fragments in the promoter regions of GmERF13a h, GmERF13b i, GmERF13c j, and GmERF13d k in empty vector (pS1300), GmABI5aOX, and GmABI5bOX transgenic hairy roots. Data are presented as means ± SD from three biological repeats. Asterisks represent statistically significant differences from empty vector (pS1300). Two-sided Student’s t test: *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001. The exact P values are noted in the Source data. Source data are provided as a Source Data file.
