Fig. 9: GmERF13s mediate salt stress inhibition of nodulation.

a Nodulation phenotypes of W82 and GmERF13s CRISPR/Cas9 mutants at 28 dpi under normal conditions (0 mM NaCl) or salt stress conditions (75 mM NaCl). Scale bar, 1 cm. b Nodule number/dry weight per plant of W82 and GmERF13s CRISPR/Cas9 mutants after 28 dpi under normal condition (0 mM NaCl) or salt stress condition (75 mM NaCl). Different numbers indicate the decrease percentage of NaCl treatment. Data are presented as means ± SD from three biological repeats. 10 plants were collected for analysis in each repeat. c Nodulation phenotypes of empty vector (pS1300) and GmERF13s^OX transgenic hairy roots at 28 dpi under normal conditions (0 mM NaCl) or salt stress condition (75 mM NaCl). Scale bar, 1 cm. d Nodule number per hairy root of empty vector (pS1300) and GmERF13s^OX after 28 dpi under normal condition (0 mM NaCl) or salt stress condition (75 mM NaCl). Different numbers indicate the decrease percentage of NaCl treatment. Data are presented as means ± SD from three biological repeats. 18 hairy roots were collected for analysis in each repeat. e Relative expression levels of GmERF13s by RT-qPCR in transgenic hairy roots expressing empty vector (pS1300) or GmERF13s^OX after 28 dpi under normal condition (0 mM NaCl) or salt stress condition (75 mM NaCl). All hairy roots of empty vector (pS1300) or GmERF13s^OX were divided into three mixed samples for detecting, respectively. Data are presented as means ± SD from three biological replicates. Asterisks represent statistically significant differences relative to the empty vector (pS1300). Two-sided Student’s t test: *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001. The boxes in (b, d) indicate the first and third quartiles, and the whiskers indicate the minimum and maximum values. The lines within the boxes indicate the median values. The exact P values are noted in the Source data. Source data are provided as a Source Data file.