Fig. 3: Characterization of NIR CL in vitro. | Nature Communications

Fig. 3: Characterization of NIR CL in vitro.

From: Molecular Engineering of Direct Activated NIR-II Chemiluminescence Platform for In Vivo Chemiluminescence-fluorescence Duplex Imaging

Fig. 3

A Proposed response and luminescence mechanism. B Absorbance spectra of CL-O1, CL-O2, FL-O1, and FL-O2 in DCM. C CL spectra of CL-O1 and CL-O2 in the presence of TBAF in DCM. D FL spectra of FL-O1 and FL-O2 in the presence of TBAF in DCM (the excitation wavelength of FL-O1 is 520 nm and that of FL-O2 is 550 nm). E Absorbance spectra of CL-O3, CL-S3, CL-Se3, FL-O3, FL-S3, and FL-Se3 in DCM. F CL spectra of CL-O3, CL-S3, and CL-Se3 in the presence of TBAF in DCM. G FL spectra of FL-O3, FL-S3, and FL-Se3 in the presence of TBAF in DCM (the excitation wavelength of FL-X3 is 808 nm). H CL kinetic profiles of CL-O3, CL-S3, and CL-Se3 in the presence of TBAF. I CL enhancements of CL-O3, CL-S3, and CL-Se3 after treatment with different kinds of ions. J CL intensities of CL-X3 (X = O, S, or Se) as a function of [F–] (0–200 μM). Data are the mean ± SD; n = 3 independent experiments.

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