Fig. 1: Hemocytes contain both a lamellar and cortical actin network.
From: Moesin integrates cortical and lamellar actin networks during Drosophila macrophage migration
a Deformation map of the lamellar actin retrograde flow field highlighting the increase in actin network deformation around the cell body (arrowheads). b Analysis of the negative divergence in the actin retrograde flow field revealing network compression and actin accumulation around the hemocyte cell body (arrowheads). Only negatively divergent regions are highlighted. a.u., arbitrary units. c Quantification of negative divergence at the lamellar and cortical regions. ****P < 0.0001, Mann–Whitney two-tailed test. Boxplot shows medians, 25th and 75th percentiles as box limits, minimum and maximum values as whiskers; each datapoint is displayed as a marker (n = 13 hemocytes for each genotype). d 3D reconstruction of a hemocyte highlighting the transition between the flat lamellar network (arrows) and the rounded cell body (dashed circle). e Localization of Moesin and PIP2 (PLC-PH-GFP) to the region surrounding the hemocyte cell body (arrowheads). f Ratiometric imaging of a membrane-proximal actin probe (MPAct) with an actin (F-tractin) or membrane probe (CaaX) highlighting an increase in membrane-associated actin around the hemocyte cell body (arrowheads). Scale bars, 10 µm.
