Fig. 5: In vivo fluorescence imaging and tumor inhibition of HTPSNiclo-mediated photo-immunotherapy.
From: Hypoxia-tolerant polymeric photosensitizer prodrug for cancer photo-immunotherapy
NIR fluorescence imaging (a) and MFI b of 4T1 tumor-bearing BALB/c mice after intravenous injection of HTPS and HTPSNiclo at different time points (200 µL, [BTP-OD] = 200 μg/mL) (n = 3 independent experiments). c Quantitative analysis of DHR123 MFIs in primary tumor tissues from mice in different group. NIR irradiation: 0.2 W/cm2 at 808 nm for 5 min (n = 3 independent experiments). ***p = 6.5 × 10-4 HTPS versus saline, ***p = 2.8 × 10-4 HTPSNiclo versus saline. d Schedule for remote tumor model implantation and HTPSNiclo-mediated photo-immunotherapy. Growth curves of e primary tumors and f distant tumors on 4T1 remote tumor model (n = 5 independent mice). **p = 1.1 × 10−3 HTPSNiclo + NIR versus HTPS + NIR in (e), ****p = 4.3 × 10−5 HTPSNiclo + NIR versus HTPS + NIR in (f). g Histological H&E staining of lung in 4T1 tumor-bearing mice after different treatment. Images are representative of three biologically independent mice (Scale bar: 500 μm). h Tumor metastasis area in lungs of mice in 4T1 tumor-bearing mice after different treatment. **p = 2.9 × 10−3 HTPSNiclo versus saline, *p = 1.2 × 10−2 HTPS + NIR versus saline, **p = 1.1 × 10−3 HTPSNiclo + NIR versus saline. i Survival curves for the mice after different treatments (n = 5 independent mice). j Schedule for rechallenged tumor model establishment and HTPSNiclo-mediated photo-immunotherapy. k Growth curves of rechallenged tumors (n = 5 independent mice). ****p = 1.4 × 10−5 HTPSNiclo + NIR versus saline. l Number of lung metastasis nodules after different treatments on rechallenged tumor model (n = 3 independent experiments). ****p = 4.1 × 10−5 HTPSNiclo + NIR versus saline. m Images of lung metastasis after staining with India ink. Statistical significance was calculated via one-way ANOVA with a Tukey posthoc test. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. The mean values and SD are presented. Source data are provided as a Source Data file.
