Fig. 3: Characterization of AuraA. | Nature Communications

Fig. 3: Characterization of AuraA.

From: Characterization and structural analysis of a versatile aromatic prenyltransferase for imidazole-containing diketopiperazines

Fig. 3

a Phylogenetic tree analysis of AuraA. Representative DMATS, UbiA, NphB/CloQ, and peptide PT form four clades, indicated by blue, orange, purple, and green background, respectively. AuraA is highlighted in red. b Purification of AuraA as a His12-tagged fusion protein post gene expression. Lane 1: purified His12-AuraA; lane 2: molecular mass standard. This experiment was repeated independently three times. c LC-MS traces from in vitro assays with AuraA using substrate 2 or 3. X5 represents that the trace values of m/z 237 have been amplified fivefold. Source data are provided as a Source Data file.

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