Fig. 3: Fitness of promastigote mutants in vitro.
A Growth curve of the mutant pool. Measurements for each of the three replicate samples are plotted individually. DNA was sampled at the time-points highlighted by a blue circle. Source Data are provided as a Source Data file. B Small grey dots indicate TransLeish mutant cell lines, ranked in order of their fitness score, from lowest to highest. Larger dots show parental control cell lines (SBL1-5) and control mutants, as labelled; The source data are provided in Supplementary Data 4. C Doubling times of the parental control line (turquoise dots), the slow growing control knockout cell line ΔIFT88 (LmxM.17.1230) and selected transporter knockout mutants are plotted. Each dot represents the average doubling time of three replicates for a 24h-interval. The bars indicate mean and standard deviation for each cell line. Source Data are provided as a Source Data file. Asterisks indicate mutants with a significantly increased doubling time compared to the parental (unpaired two-tailed t-test): ΔLmxM.28.0350 (Sweet2) p = 0.4019, Δ LmxM.13.1210 (NT3) p = 0.5612, Δ LmxM.14.1355 (FT) p = 0.7030, Δ LmxM.08_29.0650 (Pho89) p = 0.9338, Δ LmxM.14.1460 (MPC2) p = 0.0737, Δ LmxM.21.1340 (V-ATPase V1 H) p = 0.0077, Δ LmxM.17.1230 (P-ATPase) p = 0.000087, ΔLmxM.12.0520 (V-ATPAse V1 F) p = 6 x 10-06, ¥LmxM.33.3060 (MCP6) p = 2 × 10-08, ¥¥LmxM.10.1020 (MCP19) p = 2.4 × 10-11, ΔLmxM.08_29.2780 (MIT1) p = 3.2×10-12, ΔLmxM.01.0810 (CAKC) p = 9.9 × 10-9, Δ LmxM.31.2660 (AAP7) p < 10-15, ΔLmxM.19.0310 (GDT1) p = 1.3×10-5, Δ LmxM.31.1110 (MCP2) p = 3.4 × 10-6, ΔLmxM.32.3040 (ABCI3) p = 3.4 × 10-7. Mutants where gene deletion was inconclusive (¥) or incomplete (¥¥) are indicated.
