Fig. 2: seRNA targeting specificity.
From: Selectively expressed RNA molecules as a versatile tool for functionalized cell targeting
Using keratin as a selectively expressed target for seRNA activation, keratin-negative human foreskin fibroblasts (HFF) and primary neurons, as well as keratin-positive breast cancer (MCF-7) and glioblastoma cell lines (exemplarily shown for SF188) were stained. Scale bar = 50 µm. a Non-selective and selectively expressed RNA constructs with indicated domain architecture were used for expression analysis in non-target and target cells after transfer as DNA-expression plasmid (b, c). Flow cytometry-based eGFP positive cells were either given as a percentage of all cells (b, c)) or as relative value +/− s.d. using transfection efficiencies of non-selectively expressed RNA constructs as reference. P-values of 0.01 based on a one-way ANOVA test are indicated by two stars (d). n = at least 3 independent experiments for (b–d). Flow-cytometry analyses of primary cortical neurons (top) and U87 glioblastoma cells (bottom) expressing non-selective and seRNA constructs. The threshold (red line) indicates 99% of all cells in an untransfected control (e).
